Creating Tools to Determine Whether Katanin 60 Affects Female Rejection of Males in Drosophila

During courtship, it is vital for organisms to recognize conspecifics because of the costs associated with forming interspecies hybrids. Many organisms use species-specific cues to recognize potential mates. These cues are perceived and evaluated via neural pathways. The genetic basis of how species-specific cues are evaluated and processed into receptive or rejection behaviour remains almost entirely unknown. The gene Katanin 60 (Kat60) has previously been identified as contributing to interspecific mate rejection between Drosophila melanogaster and D. simulans. I use the CRISPR/Cas9 system and RNA interference (RNAi) to confirm if Kat60 influences female receptivity between D. melanogaster and D. simulans and to identify which tissues affect it. I have created 16 RNAi stocks that silence transcripts of one species’ allele, and one CRISPR stock that disrupts the D. melanogaster Kat60 sequence. These stocks can be used in future reciprocal hemizygosity experiments to determine if Kat60 affects interspecific mate rejection

A novel mistranslating tRNA model in Drosophila melanogaster has diverse, sexually dimorphic effects

Transfer RNAs (tRNAs) are the adaptor molecules required for reading the genetic code and producing proteins. Transfer RNA variants can lead to genome-wide mistranslation, the misincorporation of amino acids not specified by the standard genetic code into nascent proteins. While genome sequencing has identified putative mistranslating transfer RNA variants in human populations, little is known regarding how mistranslation affects multicellular organisms. Here, we create a multicellular model of mistranslation by integrating a serine transfer RNA variant that mistranslates serine for proline (tRNAUGG,G26ASer) into the Drosophila melanogaster genome. We confirm mistranslation via mass spectrometry and find that tRNAUGG,G26ASer misincorporates serine for proline at a frequency of ∼0.6% per codon. tRNAUGG,G26ASer extends development time and decreases the number of flies that reach adulthood. While both sexes of adult flies containing tRNAUGG,G26ASer present with morphological deformities and poor climbing performance, these effects are more pronounced in female flies and the impact on climbing performance is exacerbated by age. This model will enable studies into the synergistic effects of mistranslating transfer RNA variants and disease-causing alleles

Orbital Orientations of Exoplanets: HAT-P-4b is Prograde and HAT-14b is Retrograde

We present observations of the Rossiter-McLaughlin effect for two exoplanetary systems, revealing the orientations of their orbits relative to the rotation axes of their parent stars. HAT-P-4b is prograde, with a sky-projected spin-orbit angle of λ = –4.9 ± 11.9 deg. In contrast, HAT-P-14b is retrograde, with λ = 189.1 ± 5.1 deg. These results conform with a previously noted pattern among the stellar hosts of close-in giant planets: hotter stars have a wide range of obliquities and cooler stars have low obliquities. This, in turn, suggests that three-body dynamics and tidal dissipation are responsible for the short-period orbits of many exoplanets. In addition, our data revealed a third body in the HAT-P-4 system, which could be a second planet or a companion star

Hot Stars With Kepler Planets Have High Obliquities

It has been known for a decade that hot stars with hot Jupiters tend to have high obliquities. Less is known about the degree of spin-orbit alignment for hot stars with other kinds of planets. Here, we re-assess the obliquities of hot Kepler stars with transiting planets smaller than Neptune, based on spectroscopic measurements of their projected rotation velocities (vsini). The basis of the method is that a lower obliquity -- all other things being equal -- causes sini to be closer to unity and increases the value of vsini. We sought evidence for this effect using a sample of 150 Kepler stars with effective temperatures between 5950 and 6550K and a control sample of 101 stars with matching spectroscopic properties and random orientations. The planet hosts have systematically higher values of vsini than the control stars, but not by enough to be compatible with perfect spin-orbit alignment. The mean value of sini is 0.856 +/- 0.036, which is 4-sigma away from unity (perfect alignment), and 2-sigma away from pi/4 (random orientations). There is also evidence that the hottest stars have a broader obliquity distribution: when modeled separately, the stars cooler than 6250K have = 0.928 +/- 0.042, while the hotter stars are consistent with random orientations. This is similar to the pattern previously noted for stars with hot Jupiters. Based on these results, obliquity excitation for early-G and late-F stars appears to be a general outcome of star and planet formation, rather than being exclusively linked to hot Jupiter formation.Comment: AJ, in press [15 pages

Planet Candidates from K2 Campaigns 5-8 and Follow-Up Optical Spectroscopy

We present 151 planet candidates orbiting 141 stars from K2 campaigns 5-8 (C5-C8), identified through a systematic search of K2 photometry. In addition, we identify 16 targets as likely eclipsing binaries, based on their light curve morphology. We obtained follow-up optical spectra of 105/141 candidate host stars and 8/16 eclipsing binaries to improve stellar properties and to identify spectroscopic binaries. Importantly, spectroscopy enables measurements of host star radii with $\approx$10% precision, compared to $\approx$40% precision when only broadband photometry is available. The improved stellar radii enable improved planet radii. Our curated catalog of planet candidates provides a starting point for future efforts to confirm and characterize K2 discoveries.Comment: Accepted for publication in the Astronomical Journal; 17 pages, 8 figures, 2 tables, download source for full table

Two Transiting Earth-size Planets Near Resonance Orbiting a Nearby Cool Star

Discoveries from the prime Kepler mission demonstrated that small planets (< 3 Earth-radii) are common outcomes of planet formation. While Kepler detected many such planets, all but a handful orbit faint, distant stars and are not amenable to precise follow up measurements. Here, we report the discovery of two small planets transiting K2-21, a bright (K = 9.4) M0 dwarf located 65$\pm$6 pc from Earth. We detected the transiting planets in photometry collected during Campaign 3 of NASA's K2 mission. Analysis of transit light curves reveals that the planets have small radii compared to their host star, 2.60 $\pm$ 0.14% and 3.15 $\pm$ 0.20%, respectively. We obtained follow up NIR spectroscopy of K2-21 to constrain host star properties, which imply planet sizes of 1.59 $\pm$ 0.43 Earth-radii and 1.92 $\pm$ 0.53 Earth-radii, respectively, straddling the boundary between high-density, rocky planets and low-density planets with thick gaseous envelopes. The planets have orbital periods of 9.32414 days and 15.50120 days, respectively, and have a period ratio of 1.6624, very near to the 5:3 mean motion resonance, which may be a record of the system's formation history. Transit timing variations (TTVs) due to gravitational interactions between the planets may be detectable using ground-based telescopes. Finally, this system offers a convenient laboratory for studying the bulk composition and atmospheric properties of small planets with low equilibrium temperatures.Comment: Updated to ApJ accepted version; photometry available alongside LaTeX source; 10 pages, 7 figure

60 Validated Planets from K2 Campaigns 5-8

We present a uniform analysis of 155 candidates from the second year of NASA's $K2$ mission (Campaigns 5-8), yielding 60 statistically validated planets spanning a range of properties, with median values of $R_p$ = 2.5 $R_\oplus$, $P$ = 7.1 d, $T_\mathrm{eq}$ = 811 K, and $J$ = 11.3 mag. The sample includes 24 planets in 11 multi-planetary systems, as well as 18 false positives, and 77 remaining planet candidates. Of particular interest are 18 planets smaller than 2 $R_\oplus$, five orbiting stars brighter than $J$ = 10 mag, and a system of four small planets orbiting the solar-type star EPIC 212157262. We compute planetary transit parameters and false positive probabilities using a robust statistical framework and present a complete analysis incorporating the results of an intensive campaign of high resolution imaging and spectroscopic observations. This work brings the $K2$ yield to over 360 planets, and by extrapolation we expect that $K2$ will have discovered $\sim$600 planets before the expected depletion of its on-board fuel in late 2018.Comment: 33 pages, 13 figures, 5 tables, accepted for publication in A

Updated Parameters and a New Transmission Spectrum of HD 97658b

Recent years have seen increasing interest in the characterization of sub-Neptune-sized planets because of their prevalence in the Galaxy, contrasted with their absence in our solar system. HD 97658 is one of the brightest stars hosting a planet of this kind, and we present the transmission spectrum of this planet by combining four Hubble Space Telescope transits, 12 Spitzer/IRAC transits, and eight MOST transits of this system. Our transmission spectrum has a higher signal-to-noise ratio than those from previous works, and the result suggests that the slight increase in transit depth from wavelength 1.1–1.7 μm reported in previous works on the transmission spectrum of this planet is likely systematic. Nonetheless, our atmospheric modeling results are inconclusive, as no model provides an excellent match to our data. Nonetheless, we find that atmospheres with high C/O ratios (C/O ≳ 0.8) and metallicities of ≳100× solar metallicity are favored. We combine the mid-transit times from all of the new Spitzer and MOST observations and obtain an updated orbital period of P = 9.489295 ± 0.000005, with a best-fit transit time center at T₀ = 2456361.80690 ± 0.00038 (BJD). No transit timing variations are found in this system. We also present new measurements of the stellar rotation period (34 ± 2 days) and stellar activity cycle (9.6 yr) of the host star HD 97658. Finally, we calculate and rank the Transmission Spectroscopy Metric of all confirmed planets cooler than 1000 K and with sizes between 1 R⊕ and 4 R⊕. We find that at least a third of small planets cooler than 1000 K can be well characterized using James Webb Space Telescope, and of those, HD 97658b is ranked fifth, meaning that it remains a high-priority target for atmospheric characterization